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KMID : 1005220100250030165
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Journal of Embryo Transfer
2010 Volume.25 No. 3 p.165 ~ p.169
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Effect of Supplementation of Vitamin E on In Vitro Maturation and Activation of Bovine Oocytes
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Park Jong-Im
Jang Yuni
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Abstract
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This study was carried out to assess the effect of vitamin E against the reactive oxygen species (ROS) on chemical activation of in vitro matured oocytes. Bovine oocytes were aspirated from slaughtered ovaries and transferred to maturation medium with or without vitamin E (). After 22 hours of culture, oocytes with polar bodies were selected and submitted to activation treatments with or without vitamin E. After activation, oocytes were cultured in mSOF medium and rate of development was monitored. For ROS () detection, in vitro matured and activated oocytes were selected and stained with DCFDA and observed under fluorescence microscope. The ROS contents were not significant differences in IVM rate, activation process and embryonic development to blastocysts with or without vitamin E. The cell number of blastocyst showed significant difference (p<0.05) in embryos matured and activated with vitamin E. The results of the present study demonstrated that the exposure of vitamin E in IVM and activation process improved the quality of embryos evaluated by the cell number of blastocysts.
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KEYWORD
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ROS, vitamin E, bovine, oocyte
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